Amphoteric actives are surfactants that can ionize in both acidic and basic environments and are often found in cleaning products, shampoos, and some personal care products. Amphoteric actives are used to maintain the pH balance of the skin, increase the softness of products and create synergistic interactions with other surfactants.

The determination of the amphoteric active substance is used to measure the concentration of this component in the product. Below is a general procedure for the determination of the amphoteric active substance:

1. **Sample Preparation**: A certain volume of the liquid to be analyzed is taken and diluted with a certain volume of solvent.
2. **Extraction**: If necessary, a suitable extraction method is used to extract the amphoteric active substance from the sample.
3. **Titrimetry**: Determination of amphoteric active substance can be performed by acid-base vibration. This means titrate the amphoteric substance using an acid or base at a known concentration. After the end point is determined, the amount of acid or base used is used to calculate the amphoteric active substance concentration.
4. **Spectrophotometry**: In some cases, UV-vis spectrophotometry or other spectroscopic methods can be used to determine the amphoteric active substance.
5. **Evaluation of Results**: Obtained results are compared to product specifications or standards.

This procedure is a general approach and specific requirements and protocols may be required for a specific product or substance. The method used for the determination of the amphoteric active substance may vary depending on the product analyzed, the equipment available, and other factors.